Histological analysis showed that most of these macrophage‐depleted recipients had pristine allograft coronary arteries with no evidence of vasculopathy (Figure 3A), a notable difference from the aspirin‐only controls (Figure 3B). From these area values the ‘neointimal index’, defined as [intimal area/(luminal + intimal area)]× 100, was calculated. Working off-campus? A small molecule (AP20187) was administrated to … Macrophages activated ex vivo or macrophages from transgenic mice can be adoptively transferred during disease models to determine whether a specific protein or activation state affects disease outcome. We thank Dr. Bruce Rosengard for critical review of this manuscript. These confounding immune defects prevented the definitive interpretation of transplant experiments utilizing op/op mice, leaving the actual role of macrophages in CAV pathogenesis unresolved. II. The link between major histocompatibility complex antibodies and cell proliferation. Effects of different serotonin receptor subtype antagonists on the development of cardiac allograft vasculopathy in murine aortic allografts. Morphometric analysis, similar to what has been described previously (18), was performed on images of coronary arteries near the ostia on tissue sections stained with Weigert's elastic stain. | Please check your email for instructions on resetting your password. Macrophage facilitation of the adaptive immune alloresponse is also promoted by production of chemokines such as RANTES/CCL5 and MIG/CXCL9. injections of gadolinium(III) chloride (10 mg/kg body weight) on preoperative day ‐2 and weekly thereafter (12, 15). All animals were cared for according to American Association for the Accreditation of Laboratory Animal Care approved methods. Bone marrow-derived macrophages (BMDMs); Clodronate liposomes; Macrophage activation; Macrophage colony-stimulating factor (MCSF); PBS liposomes; PKH26. To understand the role of macrophages during LC, liposomal clodronate was used to deplete macrophage levels before traumatic insult. Could a B-1 cell derived phagocyte "be one" of the peritoneal macrophages during LPS-driven inflammation? National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Macrophages were depleted in a murine heterotopic cardiac transplant system known to develop fulminant CAV lesions. The success of this strategy was correlated with the degree of macrophage depletion achieved. Transplanted hearts continued to beat vigorously up to the day of sacrifice and showed no signs of acute rejection (Table 1). Innate immune mechanisms in transplant allograft vasculopathy. A p‐value of <0.05 was considered significant. Transplanted hearts were typically removed from recipients on postoperative day 31 and frozen sections were prepared to determine the presence and degree of CAV, as previously described (16). The op/op mouse, however, also suffers from pleiotrophic immune defects such as aberrant granulocyte function, impaired Th1 differentiation and irregular B‐cell lymphopoeisis (27-29). The Significance of Non–T-Cell Pathways in Graft Rejection: Implications for Transplant Tolerance. Hua L(1), Shi J(1), Shultz LD(1), Ren G(2). Keywords: Significant differences between group means were determined using the Fisher's exact test. Macrophages may represent the end‐effector cells in a final common pathway towards CAV independent of T‐cell or B‐cell alloreactivity and exert their injurious effects through mechanisms related to cytokine/growth factor production rather than phagocytosis. Depletion of Cells of Monocyte Lineage Prevents Loss of Renal Microvasculature in Murine Kidney Transplantation.
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